PSXII-5 Evaluation of Influence of Single-Nucleotide Polymorphism of CSN3 on Amino Acid and Mineral Composition of Milk from the Holstein Cows

Abstract The aim was to study the dependence of single-nucleotide polymorphism CSN3 gene on amino acid and mineral composition milk Holstein cows. object research is cows (n=100; age - 4-5 years). elemental estimated according 25 chemical elements (Al, As, B, Ca, Cd, Co, Cr, Cu, Fe, I, K, Li, Mg, Mn, Na, Ni, P, Pb, Se, Si, Sn, Hg, Sr, V, Zn) by ICP AES MS. (arginine, tyrosine, phenylalanine, hiscidin, leucine-isoleucine, methionine, valine, proline, threonine, serine, alanine, glycine) analyzed high performance liquid chromatography using a cation exchange analyzer (Knauer, Germany) subsequent post-column derivatization with ninhydrin. Single nucleotide K-casein determined PCR analysis in whole blood samples. distribution animals genotypes locus as follows: AA–51 heads, BB–38 heads; AB–11 goals. results studies showed that AA genotype relation AB BB contained more lysine (g/100 g protein) 5.4 8.6 (Р≤0.05)%, methionine 6.8 (Р≤0.05) 9.2% (Р≤0.05), arginine 4.7 10% (Р≤0.05). In genotype, greater concentrations essential (Se, Zn, Mg) minimal toxic Pb Cd towards genotypes. maximum amount Sr V significant difference genotype. Thus, it can be concluded compositions are significantly interrelated polymorphism.